A Comparison of Two Methods for Collecting Hemolymph of Musca domestica (Housefly) Larvae
［Key words］ houseflies; larvae ; hemolymph
To compare two methods for collecting hemolymph of housefly larvae. Methods: Two methods, beheading and transient bodywall breaking down (TBB), were compared by comparing the total hemolymph volume, protein concentration and protein bands on SDS-PAGE jell of collections respectively by the two methods. Housefly larvae in two states: heat induced and non-induced were used in each method experiment. Results: The hemolymph protein concentration of heat-induced larvae collected by beheading was higher than that of non-induced larvae; The hemolymph protein concentration collected by TBB method was higher than that collected by beheading method; The bands on SDS-PAGE jell of hemolymph collected by TBB method were more than those collected by beheading method. Conclusion: TBB method is more simple and convenient for collecting the housefly larvae hemolymph, and the hemolymph protein ingredients are richer than those collected by beheading method.
