Relationship between Glutathione S-transferase Genetic Polymorphism and Glutathione S-transferase Activity in Arsenism Caused by Coal-buring
［Key words］arsenic poisoning; glutathione S-transferase genetic polymorphisms; glutathione S-transferase
To explore the relationship between glutathione S-transferase (GST)genetic polymorphisms and GST activity in coal-buring caused arsenism and provide a scientific basis for a more in-depth explanation to arsenism mechanism. Methods: One hundred ant thirty arsenism patients and 140 healthy people were chosen as subjects of the study. Multiple polymerase chain reaction (multiplex PCR) was used to detect deletion of GSTM1, and GSTT1. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique was used to detect polymorphisms of GSTP1, Ile105Val, GSTO1 Ala140Asp, and GSTO2 Asn142Asp. Chemistry chromatometry method was used to detect GST activity. The relationship between the genetic polymorphisms and GST activity was analyzed. Results: The frequencies of GSTT1(-/-) , GSTT1(+/+)or(+/-) and genotypes Asn/Asn, Asn/Asp and Asp/Asp of gene GSTO2 Asn142Asp were significantly different between arsenism group and control group（P<0.05）. GST activity of arsenism patients increased when compared with that of controls. The difference was statistically significant（P<0.05）. GST activity of different genotypes of GSTO1 Ala140Asp was different between arsenism group and control group. Conclusion: Gene polymorphism of GSTO1 Ala140Asp may relate to GST activity of arsenism patients caused by coral-burning. Further research on function and transcription regulation of the gene is needed.
