The Inhibitory Effect of Sodium Ferulate on the Expression of PAI-1 and p38MAPK Induced by High Glucose in Mesangial Cells
［Key words］ diabetic nephropathies; glucose; mitagen-activated protein kinases; plasminogen activator inhibitor 1; mesangial cell; sodium ferulate
To observe the influence of sodium ferulate (SF) on PAI-1 and p38MAPK protein expression of mesangial cells in incubation medium with high glucose. Methods: The primarily cultured glomerular mesangial cells (GMCs) were divided into normal glucose group, mannitol group, high glucose group and high glucose plus SF groups of which cells exposed to different concentrations of SF. Cells were collected in 24 h and 48 h after being treated with corresponding agents respectively. The expression of PAI-1 was detected with flow-cytometry and that of p38MAPK protein was detected with immunocytochemistry methods. Results: High glucose significantly promoted the expression of PAI-1 and p38MAPK of GMCs when cultured for 24 h and 48 h. SF inhibited these promotion of PAI-1 and p38MAPK protein induced by high glucose, and the inhibitory effect increased along with the increase of SF concentration and the extension of its affecting time. Conclusion: SF can inhibit the increase of PAI-1 protein and the activation of p38MAPK signal pathway of GMCs induced by high glucose .
