The Renal Lesions and SnoN Protein Expression in Kidney Tissue of Rats with Type 2 Diabetes

     ［Key words］ diabetes mellitus，non-insulin-dependent; diabetic nephrosis； rats,Sprague-Dawley；transcription co-repressor SnoN
     To establish type 2 diabetic rat model and observe its renal lesions and SnoN protein expression. Methods: Sprague-Dawley rats were divided into normal control, high-fat and high-sucrose groups. High-fat and high-sucrose groups were then divided into high-fat and high-sucrose controls and diabetes mellitus groups after taking high-fat and high-sucrose diet for 12 weeks. The diabetic rats were injected with low dose of streptozotocin (30 mg/kg) and were killed at the 20th, 24th, and 28th weeks respectively. The SnoN proteins in renal cortex were detected with Western blotting. Blood glucose, serum insulin concentration, trigly-ceride, total cholesterol, serum creatinine and 24 h urine protein were examined with biochemistry methods. The renal morphology on HE stained sections was checked with light microscope. Results: Diabetic rats showed hyperglycaemia, hyperlipemia, kidney morphological lesion and renal function changes. High-sucrose control group showed hyperinsulinemia and hyperlipemia, but the blood glucose was normal. Western blotting results showed that the expression of SnoN in diabetic renal tissues significantly decreased (P<0.01). Conclusions: Rat models of type 2 diabetes mellitus have been established successfully which can be used as a diabetic nephropathy model. The change of SnoN protein expression may play a role in the mechanisms of type 2 diabetic nephropathy.