An Experimental Study on the Protective Effect of Whole-body Hypoxic Preconditioning on Lung Injury Caused by Ischemic Reperfusion in Rats
［Key words］ ischemia; lungs; reperfusion injury; hypoxic preconditioning; rats，sprague-dawley
To investigate the protective effect of hypoxic preconditioning (HPC) on lung ischemia-reperfusion injury in rats. Methods: HPC model was established with 5 times of repetitive sublethal hypoxia. Thirty rats were randomly divided into the following three groups (n=10 in each): control group, ischemia-reperfusion injury group (group I/R), and group HPC+I/R. I/R injury model rats underwent 45 min of left hilar ligation followed by 120 min of reperfusion. The ratio of wet/dry weight of lung tissue was measured and calculated. The contents of malondialdehyde (MDA), and activities of superoxide dismutase (SOD) and myeloperoxidase (MPO) in lung tissue were determined with spectrophotometry. Results: In group I/R,the wet/dry weight ratio of lung was significantly higher than that of control group (P<0.05), and the MDA content and MPO activity in lung tissue were dramatically higher (P<0.05), SOD activity was significantly lower (P<0.01) than those of control group. In group HPC+I/R, that rats subjected to HPC, these oxidative stress indexes were significantly improved when rats exposed to ischemia-reperfusion.Conclusion: Whole-body hypoxic preconditioning in rats can protect lung from I/R injury by reducing oxidative stress.
