Establishment and Optimization of SRAP-PCR Amplification System for Housefly
［Key words］ Muscidae; random amplified polymorphic DNA technique; genetic markers
To establish and optimize SRAP-PCR reaction system of housefly. Methods: Effects of concentrations of template DNA, Mg2+, dNTPs, primers and amount of Taq DNA polymerase on SRAP molecular marker amplification system of Musca domestica L were studied in monofactorial experiment, and the amplification system was optimized. Results: Total volume of the optimal system was 30μl containing 50 ng of template DNA, 1.5mmol/ L of Mg2+, 0. 20 mmol/ L of dNTPs, 0. 15μmol/ L of primers and 0. 50 U of Taq DNA polymerase. Conclusion: This system provides a useful tool for analysis of genetic diversity and gene location of Musca domestica L.
To establish and optimize SRAP-PCR reaction system of housefly. Methods: Effects of concentrations of template DNA, Mg2+, dNTPs, primers and amount of Taq DNA polymerase on SRAP molecular marker amplification system of Musca domestica L were studied in monofactorial experiment, and the amplification system was optimized. Results: Total volume of the optimal system was 30μl containing 50 ng of template DNA, 1.5mmol/ L of Mg2+, 0. 20 mmol/ L of dNTPs, 0. 15μmol/ L of primers and 0. 50 U of Taq DNA polymerase. Conclusion: This system provides a useful tool for analysis of genetic diversity and gene location of Musca domestica L.
