［Key words］ colonic neoplasma; cell cycle; photodynamic therapy; proliferation
To study the expression of correlation factors of P21, P27, cyclinE, and CDK2 in colon carcinoma SW480 cells after the treatment of 5-aminolevulinate acid photodynamic therapy (ALA-PDT). Methods: The expression of P21, P27, cyclinE, and CDK2 in colon carcinoma cell line (SW480 cells) were detected by using reverse transcriptase-polymerase chain reaction (RT-PCR) technique after the cells were treated with ALA-PDT. Results: The expression of P21 increased (P<0.01) while the mRNA contents of cyclinE and CDK2 decreased (P<0.01) remarkably in SW480 cells after the therapy of ALA-PDA than before. Content of P27 mRNA didn’t change significantly. Conclusion: The inhibitory effects of ALA-PDT to the proliferation of colon carcinoma SW480 cell line might be caused by its up-regulation to P21 over-expression, and down-regulation to cyclinE, and CDK2.
