The Effect of Ganodermo Lucidum Triterpenoids on Catalase, Acetylcholinesterase, Superoxide Sismutase and Malondialdehyde Contents in Alzheimer Disease Rats
［Key words］ Alzheimer disease；Ganoderma; brain; catalase; acetylcholinesterase; superoxide dismutase; malondialdehyde; rat, sprague-dawley
To investigate the preventive effects of ganodermo lucidum triterpenoids (GLT) to Alzheimer disease（AD）in model rats. so as to provide theory basis for new drug exploitation and clinical application. Methods: Eighty SD rats chosen by Morris water maze test were randomly divided into 8 groups: normal control group ( group NC, received 10 ml/kg of distilled water), sham operation group (group SO, received 10 ml/kg of distilled water), model group (group M, received 10ml/kg of distilled water), GLT treated groups (group G1, received 0.25 g/kg of GLT; group G2, 0.50 g/kg, and group 3, 1.0 g/kg), masculine drug group (group MD, received 0.54 g/kg of Jiannao capsules)，and solute mediated group (group SM, received 10 ml/kg of edible oil). All of the SD rats except those in group NC and group SO were microinjected with beta-amyloid peptide segment 25-35 (Aβ25-35) into lateral ventricle to produce AD model, and instead, rats in group NC were injected with saline. GLT treatment began on the next day, once a day intragastrically for 20 days. Then, the animals were executed, and the brain tissues were taken out and blended for the tests of activities of catalase (CAT) , acetyl cholinesterase (ACHE), superoxide dismutase (SOD), and the content of malondialdehyde (MDA) with chemistry detecting kits. Results: The activities of CAT, SOD were lower, while ACHE activity and MDA content were higher in rats of group M than those in group NC; The activities of CAT, SOD were higher, while ACHE activity and MDA content were lower in GLT groups than those in group M. Conclusion: The GLT has ability of strengthening the cleaning of free radicals in Aβ25-35 toxicated rats, and ability of anti-liqid-peroxidation, and can promote the restore of choline nervous system function.
